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A cis-regulatory atlas for de-etiolation in G. gynandra The chromatin accessibility assays and in silico analysis above identified regulatory elements that could be important for gene regulation but cannot indicate whether motifs were actually bound by transcription factors. We therefore carried out sequencing at sufficient depth to define DNA sequences protected from DNase I digestion.
Abstract C4 photosynthesis evolved repeatedly from the ancestral C3 state, improving photosynthetic efficiency by ~50%. In most C4 lineages, photosynthesis is compartmented between mesophyll and bundle sheath cells, but how gene expression is restricted to these cell types is poorly understood. Using the C3 model Arabidopsis thaliana, we identified cis-elements and transcription factors driving expression in bundle sheath strands.
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