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Abstract Autophagy is a lysosome-based proteolytic system that is essential for cellular homeostasis. However, it remains unclear whether autophagy regulates the homeostasis of non-membranous organelles, such as centrosomes. We herein provide evidence to show that the depletion of centrosomes by centrinone, a PLK4 inhibitor, appears to activate autophagy. We then searched for centrosomal proteins whose loss promotes autophagy.
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