The DigiWest approach combines standardized protein separation and western blotting with a multiplexed, bead-based immunoassay platform, such as the Luminex FlexMAP 3D system. As in standard western blotting, proteins are size separated via SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a blotting membrane (Fig. 1a,b). On this supporting membrane, all separated proteins are biotinylated before one sample lane is cut horizontally into 0.5-mm wide strips (Fig. 1c).
