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SPECIAL ISSUE This article is part of the Software Tools and Resources 2021 special issue. Targeted proteomics quantification of proteins is typically performed using a triple quadrupole operated in multiple reaction monitoring (MRM) mode, or using a mass spectrometer capable of parallel reaction monitoring (PRM) like Orbitraps. (1−7) In both, an initial filtering step isolates the precursor peptide ion typically using a quadrupole filter.
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