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Articles by Stacey Willard
Microalgae Culture Using The DASGIP® PBR4 Module For Illumination With An Eppendorf Bench-Scale Stirred-Tank Bioreactor
By Stacey S. Willard, James Jarvis, Jean-François Hamel, and Ma Sha The number of bioprocess applications for microalgae has increased in recent years, particularly in the field of biofuel production. The combination of an Eppendorf bench-scale stirred-tank bioreactor and the DASGIP LED Illumination System creates a bioreactor setup which is capable of supporting high density microalgal growth.
Push-Button Simplicity: Automatic Fermentation with the BioFlo® 120 Auto-Culture Mode
Learning to use a bioprocess controller is a complex endeavor. Even if someone has previous bioprocess experience, moving to a new software platform can entail much learning and reduce process efficiency. With the auto-culture modes of the Eppendorf BioFlo 120 bioprocess control station, a user can select with a push of a button either a predefined Escherichia coli batch fermentation protocol or a Chinese hamster ovary (CHO) batch cell culture process. Proof of Concept: E.
Comparing Culture Methods in Monoclonal Antibody Production: Batch, Fed-Batch, and Perfusion
Recombinant protein manufacturing with Chinese hamster ovary (CHO) cells represents over 70% of the entire biopharmaceutical industry (1). In fact, human monoclonal antibodies (hMAbs) produced by CHO cells have played a major role in both the diagnostic and therapeutic markets for decades. One of the first human–mouse chimeric MAbs to obtain FDA approval was Roche’s rituximab treatment for non-Hodgkin’s lymphoma, chronic lymphocytic leukemia, and rheumatoid arthritis.
Successfully Cultivate Hybridoma Cells In High Densities
The Fibra-Cel packed-bed basket technology has been established as an excellent method for the growth of suspension and anchorage-dependent cell lines. The three dimensional structure of the Fibra-Cel disk provides an excellent solid-support matrix for the entrapment or attachment of animal cells, allowing constant perfusion of nutrients in a low-shear environment.
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