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Statistical analyses We generated two separate sets of data files: one with ant material only, 18 samples from 6 colonies, henceforth “Ant_Data”, and one with ant samples and corresponding nest samples taken at the same time (6 samples of each), henceforth “Ant&Nest_Data. From the sequenced data, we counted the number of reads, and the number of OTUs separately for the two data sets.
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