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Stefan Golfier

As seen in: Nature, BioRxiv
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Articles by Stefan Golfier

Dynamic switching of cell-substrate contact sites allows gliding diatoms to modulate the curvature of their paths

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By Stefan Golfier, Veikko F. Geyer, Stefan Diez, Nicole Poulsen
| BioRxiv
Abstract The directed motility of unicellular organisms is critical for their survival and ecological success, yet the mechanisms that enable rigid-walled cells to dynamically reorient and alter the shape of their trajectories remain poorly understood. Here, we investigate the gliding motility of Craspedostauros australis, a raphid pennate diatom that moves rapidly across submerged surfaces using an intracellular actomyosin motility complex and adhesive mucilage strand secretion.
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Force generation by protein-DNA co-condensation

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By Thomas Quail, Stefan Golfier, Maria Elsner, Keisuke Ishihara
| Nature Verified
Abstract Interactions between liquids and surfaces generate forces1,2 that are crucial for many processes in biology, physics and engineering, including the motion of insects on the surface of water3, modulation of the material properties of spider silk4 and self-assembly of microstructures5. Recent studies have shown that cells assemble biomolecular condensates via phase separation6.
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Cohesin and condensin extrude DNA loops in a cell-cycle dependent manner

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By Thomas Quail, Hiroshi Kimura, Jan Brugués, Stefan Golfier
| eLife
Abstract Loop extrusion by structural maintenance of chromosomes complexes (SMCs) has been proposed as a mechanism to organize chromatin in interphase and metaphase. However, the requirements for chromatin organization in these cell cycle phases are different, and it is unknown whether loop extrusion dynamics and the complexes that extrude DNA also differ. Here, we used Xenopus egg extracts to reconstitute and image loop extrusion of single DNA molecules during the cell cycle.
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