Abstract Objective: To determine the prevalence of functional limitations in the upper extremity among post-mastectomy patients, and to assess its impact on quality of life. Method: The prospective, analytical, cross-sectional study was conducted at Hayatabad Medical Complex and Khyber Teaching Hospital, Peshawar, Pakistan, from June to October 2023, and comprised female patients aged at least 18 year who had undergone unilateral mastectomy 3 months before data collection.