Abstract African swine fever virus (ASFV) is a major threat to pig production, yet its environmental distribution in Southeast Asian farming systems poorly understood. We investigated ASFV DNA in pig farm wastewater and Amblyomma javanense ticks collected from rescued Sunda pangolins in Vietnam. A polyethylene glycol (PEG)-based concentration protocol followed by p72-targeting real-time PCR (rPCR) improved analytical detection of ASFV DNA in spiked wastewater compared with direct DNA extraction.
