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Predicted sequences for Atlantic salmon (Salmo salar) slc41a1-3, cnnm1-4 and trpm6-7 transporters were retrieved from the Ssal_v3.1/GCA_905237065.2 genome release. Amino acid sequences from Atlantic salmon, a subset of teleost species representing the diversity of the group, as well as representative species from all other vertebrate groups, were aligned using CLUSTALW in Seaview (http://doua.prabi.fr/software/seaview). Ciona intestinalis and Branchiostoma floridae were used as outgroups.
Marius Takvam1*, Chris M. Wood2, 3, 4, Harald Kryvi1 and Tom O. Nilsen1* 1University of Bergen, Norway 2University of British Columbia, Canada 3University of Miami, United States 4McMaster University, Canada The final, formatted version of the article will be published soon.
1 Introduction Overfeeding, i.e., providing animals more feed than they consume, is a common practice in the aquaculture industry. The main reason is the concern of underfeeding fish, causing slow growth, low feed efficiency and increased stress levels and aggression (Cañon Jones et al., 2010; Martins et al., 2012). However, overfeeding results in release of uneaten feed into the environment, representing a significant source of pollution (Taranger et al., 2014; Ahmed et al., 2019).
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