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Rovers, S. et al. (2025) A minimally invasive transthoracic injection technique for reproducible intrapleural delivery in mice. Methods and Protocols, 8(3), 55. (doi: 10.3390/mps8030055) (PMID:40559443) (PMCID:PMC12195961) Text 359211.pdf - Published Version Available under License Creative Commons Attribution. 10MB Abstract The development of standardised, reproducible preclinical models is essential for advancing pleural mesothelioma (PM) research.
Here, we compared BAL cfDNA and plasma cfDNA as two different sources of tumor derived DNA and found that BAL cfDNA analysis is more sensitive than plasma for identifying lung cancer-derived mutations. We also explored the potential of tumor-naïve BAL cfDNA analysis for detection of lung cancer. Our results suggest that BAL cfDNA analysis could have clinical utility for identification of mutations in lung cancer patients and, potentially, for the diagnosis of lung cancer.
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