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Pushing the limits of detection for proteins secreted from single cells using quantum dots Vanessa Herrera,a Ssu-Chieh Joseph Hsu,a Maha K. Rahim,a Carol Chen,a Lisa Nguyen,a Wendy F. Liuabc and Jered B.
Single cell analysis methods are increasingly being utilized to investigate how individual cells process information and respond to diverse stimuli. Soluble proteins play a critical role in controlling cell populations and tissues, but directly monitoring secretion is technically challenging. Microfabricated well arrays have been developed to assess secretion at the single cell level, but these systems are limited by low detection sensitivity.
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