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Stable, high-resolution intravital imaging of the lung has become possible through the utilization of vacuum-stabilized imaging windows. However, this technique is extremely invasive and limited to only hours in duration. Here we describe a minimally invasive, permanently implantable window for high-resolution intravital imaging of the murine lung that allows the mouse to survive surgery, recover from anesthesia, and breathe independently.
Data on microsurgical excision of herniated lumbar intervertebral discs (LDHsurg) were obtained from the Landspitali National University Hospital (Landspitali) databases of surgical procedures (that is, lumbar microdiscectomy with surgical code ABC16 according to the NOMESCO (Nordic Medico-Statistical Committee) system)54. The so-defined phenotype is represented in the current paper by ‘LDHsurg’. Demographics of the defined sample are presented in Table 1.
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