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Conflicts of Interest The authors declare no conflicts of interest. Supporting Information Filename Description zoo70035-sup-0001-Appendix_A_Autonomy_in_Action-Empowering_Animal-Led_Choices.docx18.1 KB Appendix A Autonomy in Action- Empowering Animal-Led Choices. zoo70035-sup-0002-You_Choose_Procedure_Video.mp4109.7 MB You Choose Procedure Video. References , , , et al. 2018.
Abstract Caspase-2, one of the most evolutionarily conserved members of the caspase family, is an important regulator of the cellular response to oxidative stress. Given that ferroptosis is suppressed by antioxidant defense pathways, such as that involving selenoenzyme glutathione peroxidase 4 (GPX4), we hypothesized that caspase-2 may play a role in regulating ferroptosis.
RNA-seq analysis The total RNA quantity was measured using Qubit RNA HS (Thermo Fisher Scientific). 500 ng of total RNA was used for library preparation according to standard protocols (QuantSeq 3’mRNA-seq FWD, Lexogen). Indexed libraries were pooled and sequenced on a NextSeq500 (Illumina). A total of 5–15 million single-end 75 bp reads were generated per sample. Sequence reads were trimmed and aligned to hg38 genome using Cutadapt and HISAT2 packages. Gene counts were obtained from featureCounts.
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