Abstract Background: Sjogren’s syndrome (SS) is marked by exocrine-gland infiltration and Th17-driven inflammation; low-bioavailability Total glucosides of paeony (TGP) shows efficacy yet unclear mechanisms. Purpose: To investigate the role of intestinal mucosal immunity in SS pathogenesis and the effect of TGP on the differentiation and migration of Th17 in intestinal mucosal immunity. Methods: SS was induced in Peyer’s patches (PPs)/mesenteric lymph nodes (MLNs)-deficient mice.