Is this you? As a journalist, you can create a free Muck Rack account to customize your profile, list your contact preferences, and upload a portfolio of your best work.
Claim your profile
Body weight and body composition Body weight and discrete adipose tissue pad mass were measured using a precision scale. Whole-body composition was determined using NMR imaging (EchoMRI; Echo Medical Systems). Analysis of whole-body fat depots were performed using a 7.0 T BioSpec 70/30 horizontal animal scanner (Bruker) and quantified as previously described.80 Metabolic tests Food intake was measured in acclimatized singly-housed mice using a precision scale.
We developed a method for the analysis of the main metabolic products of utilization of glucose by isolated adipocytes. They were incubated 24 h with C-glucose. The final label distribution and cold levels of medium glucose, lactate and glycerol were estimated. Medium lactate was extracted using ion-exchange resin minicolumns prepared with centrifugation-filtering tubes in which the filter was substituted by the resin. This allowed complete washings using only 0.2 mL.
Under high-energy diets, amino acid N is difficult to dispose of, consequence of the availability of alternative substrates. We found, recently, that WAT contains a complete functional urea cycle, we analyzed the possible overall changes in WAT urea cycle (and other-related amino acid metabolism gene expressions) in rats subjected to a cafeteria diet. Adult female Wistar rats were fed control or simplified cafeteria diets.
As a journalist, you can create a free Muck Rack account to customize your profile, list your contact preferences, and upload a portfolio of your best work.
