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To evaluate if there is aberrabt activation of EZH2 in the development of OA, we compared the level of EZH2 in normal and OA human articular chondrocytes by Realtime PCR and Western blotting analysis. HE staining showed smooth surfaces in human articular cartilage and rough surfaces in OA human articular cartilage (Fig. 1A). Furthermore, Real-time PCR analysis revealed higher level of EZH2 mRNA in OA chondrocytes compared to normal chondrocytes (Fig. 1B).
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