2. Results 2.1. Method Validation The calibration curves for hexaconazole enantiomers were established by plotting the peak areas (y) against the analyte concentrations (x). Good linearities were achieved with linear equations where y = 64.33x + 1.384 and y = 63.81x + 3.016 in hexaconazole solvent, y = 56.98x − 3.691 and y = 58.22x − 0.770 in matrix-matched standards. The correlation coefficients (R2) were both above 0.99 for (+)- and (−)-hexaconazole (Figure 2).
