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Abstract: To investigate the effects of different pretreatment methods on the lipid characteristics and microstructure of Nannochloropsis oceanica , this study employed enzymatic hydrolysis, extrusion puffing, ultrasonic treatment, and microwave treatment for pretreating N. oceanica . The lipid yield, lipid composition, lipid concomitants, antioxidant activity, and microstructural changes of N. oceanica were measured before and after different pretreatments.
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