Abdoullatif Baraket

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Abstract : The early detection at low concentration, by non-invasive methods, of cardiac biomarkers in physiological fluids has attracted the interest of researchers over the last decade. This enables early diagnosis and prediction of the first signs of heart failure (HF). In this respect, the analysis of human saliva remains the most suitable medium for this non-invasive approach, as it contains a highly interesting biological matrix for general health and disease monitoring.

Abstract In this study, a novel, sensitive electrochemical enzyme-based biosensor for urea detection was presented. This biosensor combines a three-electrode system consisting of a classic Glassy Carbon Electrode (GCE) as the working electrode, a platinum counter electrode, and Ag/AgCl as the reference electrode. To construct this urea platform, a GCE was modified with a polyaniline (PANi) film. Then, bacterial urease from Proteus mirabilis was immobilized on the modified GCE (Pm-Urease-PANi-GCE).