Adrian James Thrasher on Muck Rack

Adrian James Thrasher

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Unveiling the cut-and-repair cycle of designer nucleases in human stem and T cells via CLEAR-time dPCR - Nature Communications

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By Nathan White, Sandra Wimberger, Grzegorz Sienski, Roberto Nitsch, Claire Booth, Giorgia Santilli, Alessia Cavazza, Marcello Maresca, Matthew Hebden Porteus, Adrian James Thrasher
| Nature
Abstract DNA repair mechanisms in human primary cells, including error-free repair, and, recurrent nuclease cleavage events, remain largely uncharacterised. We elucidate gene-editing related repair processes using Cleavage and Lesion Evaluation via Absolute Real-time dPCR (CLEAR-time dPCR), an ensemble of multiplexed dPCR assays that quantifies genome integrity at targeted sites.
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