Adrien Debard

Biocatalysed Synthesis of Chiral Amines: Continuous Colorimetric Assays for Mining Amine-Transaminases Léa Gourbeyre, Egon Heuson, Franck Charmantray, Virgil Hélaine, Adrien Debard, Jean-Louis Petit, Véronique de Berardinis and Thierry Gefflaut Abstract In the course of our research aimed at the design of new biocatalytic processes for the enantioselective synthesis of chiral amines, we have developed new continuous assays for the screening of amine-transaminases collections.

The biocatalytic reductive amination of ketone to chiral amine is one of the most challenging reaction. Using a genome-mining approach, we found proteins catalyzing the reductive amination of ketones without carboxylic function in α or β position. The synthesis of (4S)-4-aminopentanoic acid (ee ≥ 99.5%) was achieved with the thermoactive AmDH4 from Petrotoga mobilis in 88 % yield.