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Articles by Ailong Ke
Conversion of IscB and Cas9 into RNA-guided RNA editors
Keywords IscB Cas9 Cas13 Cmr Csm CRISPR trans-splicing A-to-I RNase Get full text access Log in, subscribe or purchase for full access. References 1. Batty, P. ∙ Mo, A.M. ∙ Hurlbut, D. ... Long-term follow-up of liver-directed, adeno-associated vector-mediated gene therapy in the canine model of hemophilia A Blood. 2022; 140:2672-2683 2. Kumar, S. ∙ Hsiao, Y.W. ∙ Wong, V.H.Y. ... Characterization of RNA editing and gene therapy with a compact CRISPR-Cas13 in the retina Proc. Natl. Acad. Sci. USA.
Assessing and engineering the IscB–ωRNA system for programmed genome editing - Nature Chemical Biology
Abstract OMEGA RNA (ωRNA)-guided endonuclease IscB, the evolutionary ancestor of Cas9, is an attractive system for in vivo genome editing because of its compact size and mechanistic resemblance to Cas9. However, wild-type IscB–ωRNA systems show limited activity in human cells.
Real-time observation of CRISPR spacer acquisition by Cas1-Cas2 integrase
Abstract Cas1 integrase associates with Cas2 to insert short DNA fragments into a CRISPR array, establishing nucleic acid memory in prokaryotes. Here we applied single-molecule FRET methods to the Enterococcus faecalis (Efa) Cas1–Cas2 system to establish a kinetic framework describing target-searching, integration, and post-synapsis events. EfaCas1–Cas2 on its own is not able to find the CRISPR repeat in the CRISPR array; it only does so after prespacer loading.
Structural basis for tRNA decoding and aminoacylation sensing by T-box riboregulators
Abstract T-box riboregulators are a class of cis-regulatory RNAs that govern the bacterial response to amino acid starvation by binding, decoding and reading the aminoacylation status of specific transfer RNAs. Here we provide a high-resolution crystal structure of a full-length T-box from Mycobacterium tuberculosis that explains tRNA decoding and aminoacylation sensing by this riboregulator.
Local-to-global signal transduction at the core of a Mn2+ sensing riboswitch
Abstract The widespread Mn2+-sensing yybP-ykoY riboswitch controls the expression of bacterial Mn2+ homeostasis genes. Here, we first determine the crystal structure of the ligand-bound yybP-ykoY riboswitch aptamer from Xanthomonas oryzae at 2.96 Å resolution, revealing two conformations with docked four-way junction (4WJ) and incompletely coordinated metal ions.
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