Aina Mogas Barcons

Article Details Mogas Barcons, A., Chari, D. M. and Adams, C. (2023) “In vitro model of neurotrauma using the chick embryo to test regenerative bioimplantation”, ALTEX - Alternatives to animal experimentation. doi: 10.14573/altex.2304171. Articles Boni, R., Ali, A., Shavandi, A. et al. (2018). Current and novel polymeric biomaterials for neural tissue engineering. Journal of Biomedical Science, 25, 90. doi:10.1186/s12929-018-0491-8 Bradbury, E. J., & Burnside, E. R. (2019).

2. Materials and Methods 2.1. Materials Cell culture reagents and cell culture grade plastics were from Thermo Fisher ScientificTM (TFS) (Loughborough, UK) or Sigma-AldrichTM (Dorset, UK) unless otherwise stated.

2. Methodology Preparation and functionalisation of CellevateTM fibres. To examine whether neural cells aligned to aligned nanofibers, we employed pre-fabricated aligned and random nanofiber scaffolds for comparison. Glass coverslips were also used to ensure that nanofibers did not affect cell viability, proliferation and differentiation from standard culture conditions. 3D-NanoMatrixTM 24-well plates with randomly distributed or aligned fibres were kindly donated by CellevateTM (Lund, Sweden).