Ajasja Ljubetic

Abstract Strategies for detecting and controlling protein interactions play a critical role in gaining insight into molecular mechanisms of biological processes and for the control of cellular processes. Conditional protein reconstitution allows control of the selected protein function based on the proximity, defined by the genetically fused domain pairs, which may be regulated by chemical or biological signals.

Polypeptides and polynucleotides are programmable natural polymers whose linear sequence can be easily designed and synthesized by the cellular transcription/translation machinery. Nature primarily uses proteins as the molecular machines and nucleic acids as the medium for the manipulation of heritable information. A protein’s tertiary structure and function is defined by multiple cooperative weak long-range interactions that have been optimized through evolution.