Ajish Kumar on Muck Rack

Ajish Kumar

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Articles

A semisynthetic Atg3 reveals that acetylation promotes Atg3 membrane binding and Atg8 lipidation

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By Membrane Biotechnology, Ajish Kumar, Yi-Tong Li, Cong Yi
| Nature Verified
Our study began with the semi-synthesis of the yeast Saccharomyces cerevisiae Atg3 K19ac-K48ac containing 310 amino acids (Fig. 1a,b). The Ser53-Ser54 bond in the N-terminal flexible loop was chosen as the ligation site17. Thus, Atg3(1-310) was divided into two segments, including chemically synthesized Atg3(1-53) peptide hydrazide and recombinant expressed Atg3(S54C-310), in which Ser54 was mutated to Cys54 for ligation.
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