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Alaa Elgaabari

As seen in: Nature, Wiley Online Library
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Articles

Enhanced HGF with increased receptor affinity and nitration-dysfunction resistance through interaction with lipoic acid trisulfide

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By Miyumi Seki, Alaa Elgaabari, Sakiho Tanaka, Jiaxi Han, Takashi Nakashima, Shoko Sawano, Wataru Mizunoya, Issei Yokoyama, Takahiro Suzuki, Judy Anderson, Kahona Zushi, Ryota Mizuochi, Kazuki Shitamitsu, Junri Miyamoto, Kaoru Mizoguchi, Reina Fujimaru, Takahiro Maeno, Ryuichi Tatsumi
| Nature Verified
Abstract Myogenic stem cell activator HGF (hepatocyte growth factor) undergoes nitration of tyrosine residues (Y198, Y250) predominantly on fast-twitch fibers to lose its binding affinity to the signaling receptor c-met, in response to peroxynitrite (ONOO-) generation during aging.
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In vitro immuno‐prevention of nitration/dysfunction of myogenic stem cell activator HGF, towards developing a strategy for age‐related muscle atrophy

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By Kyushu Fukuoka Japan, Alaa Elgaabari, Sakiho Tanaka, Miyumi Seki
| Wiley Online Library Verified
BrdU 5-bromo-2′-deoxyuridine BSA bovine serum albumin DAB 3,3′-diaminobenzidine DMEM Dulbecco's modified Eagle's medium ECL enhanced chemiluminescence Fab fragment antigen-binding Fc fragment crystallizable HGF hepatocyte growth factor HRP horseradish peroxidase HS horse serum mAb monoclonal antibody SDS sodium dodecyl sulfate TTBS tris-buffered saline Y tyrosine residue βME β-mercaptoethanol Several studies have demonstrated that HGF is a pleiotropic cytokine, displaying critical functions...
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Age‐related nitration/dysfunction of myogenic stem cell activator HGF

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By Takashi Nakashima, Alaa Elgaabari, Nana Imatomi, Hirochika Kido
| Wiley Online Library Verified
ASA accessible surface area BrdU 5-bromo-2'-deoxyuridine BSA bovine serum albumin ECL enhanced chemiluminescence ECM extracellular matrix EDL extensor digitorum longus FGF fibroblast growth factor HF highly frequent HGF hepatocyte growth factor HRP horseradish peroxidase IGF insulin-like growth factor mAb monoclonal antibody MyHC myosin heavy chain SC side chain SDS sodium dodecylsulfate TA tibialis anterior TGF transforming growth factor Y tyrosine residue βME 2-mercaptoethanol 1...
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