Alankrit Tomar

Abstract Significance Two-photon microscopy is widely used for in vivo imaging of vasculature in rodents and requires the labeling of blood plasma with fluorescent dyes such as indocyanine green (ICG). However, a major limitation of ICG is its rapid clearance from the body, which restricts its use in extended imaging sessions. We address and overcome that limitation, enabling longer in vivo imaging sessions.

Abstract We propose a technique for improving the retention time of Indocyanine Green (ICG), an FDA-approved, widely available, near infrared (NIR) probe for in vivo imaging of the cerebrovascular structure in rodents. We present a synthesis process for liposomal nanoparticles that, when used to encapsulate ICG, significantly increase the circulation time of the vascular label.

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