Highlights • ATG8 can undergo alternative conjugation to phosphatidylserine in cells • ATG8-PS occurs during non-canonical autophagy via single-membrane ATG8 conjugation • ATG8-PS can be induced by LAP, influenza A, and lysosomal ionic imbalance • ATG8-PS undergoes differential delipidation by ATG4 isoforms Summary Autophagy is a fundamental catabolic process that uses a unique post-translational modification, the conjugation of ATG8 protein to phosphatidylethanolamine (PE).
