Alfredas Bukys on Muck Rack

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Articles

Structure of the conjugation surface exclusion protein TraT

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By Ben C. Berks, Nicolas Chen, Camilla Lundgren, Alfredas Bukys
| biorxiv.org
Abstract Conjugal transfer of plasmids between bacteria is a major route for the spread of antimicrobial resistance. Many conjugative plasmids encode exclusion systems that inhibit redundant conjugation. In incompatibility group F (IncF) plasmids surface exclusion is mediated by the outer membrane protein TraT. Here we report the cryoEM structure of the TraT exclusion protein complex from the canonical F plasmid of Escherichia coli. TraT is a hollow homodecamer shaped like a chefs hat.
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