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Multiprotein collagen/keratin hydrogel promoted myogenesis and angiogenesis of injured skeletal muscles in a mouse model - BMC Biotechnology
To this end, human head hair samples were collected from local barbershops and keratin content extracted based on the previously published protocols [30, 32]. In short, 200 g human head hair samples were washed using phosphate-buffered saline (PBS) to exclude debris and contamination followed by putting them in a 37˚C oven to dry. To remove lipid content, samples were incubated in chloroform (Cat no: 1.02445.2500; Merck)/methanol (Cat no: 1.6009.2500; Merck) solution (2:1 v/v) for 24 h.
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