Alywn Dady on Muck Rack

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Articles

Engineering fluorescent reporters in human pluripotent cells and strategies for live imaging human neurogenesis

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By Lindsay Davidson, Nicolas Loyer, Timothy Sanders, Alywn Dady
| biorxiv.org
Abstract Investigation of cell behaviour and cell biological processes in human embryonic tissues is facilitated by creation of fluorescent reporters in human pluripotent stem cell lines, which can be differentiated into cell types of choice. Here we report use of a piggyBac transposon-mediated stable integration strategy to engineer human pluripotent stem cell reporter lines.
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