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Articles by Amanda Hummon
Multi‐Omic Evaluation of PLK1 Inhibitor-Onvansertib-In Colorectal Cancer Spheroids
1 Introduction The Polo gene encodes a serine/threonine kinase first discovered in Drosophila melanogaster flies in 1988 by Claudio Sunkel and David Glover [1]. A homologous kinase was mapped to humans in 1994, giving rise to the class of Polo-like kinases (Plks) [2]. There are five Plks known to exist in humans, with Plk1 being the most studied. Plk1 is activated by phosphorylation from aurora kinase A along with the cofactor Borealin [3].
Quantitative mass spectrometry imaging: therapeutics & biomolecules
Quantitative mass spectrometry imaging: therapeutics & biomolecules Mass spectrometry imaging (MSI) has become increasingly utilized in the analysis of biological molecules. MSI grants the ability to spatially map thousands of molecules within one experimental run in a label-free manner. While MSI is considered by most to be a qualitative method, recent advancements in instrumentation, sample preparation, and development of standards has made quantitative MSI (qMSI) more common.
Gas-Phase Fractionation Data-Independent Acquisition Analysis of 3D Cocultured Spheroid Tumor Model Reveals Altered Translational Processes and Signaling Using Proteomics
Download Hi-Res ImageDownload to MS-PowerPointCite This:J. Proteome Res. 2024, XXXX, XXX Terms & Conditions Most electronic Supporting Information files are available without a subscription to ACS Web Editions. Such files may be downloaded by article for research use (if there is a public use license linked to the relevant article, that license may permit other uses).
Quantification of Irinotecan in Single Spheroids Using Internal Standards by MALDI Mass Spectrometry Imaging
Skip to main page content Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation Save citation to file Format: Add to My Bibliography Your saved search Name of saved search: Search terms: Frequency: Which day? Which day? Report format: Send at most: Send even when there aren't any new results Optional text in email: Create a file for external citation management software doi: 10.1021/acs.analchem.3c00699. Online ahead of print.
Evaluating the Pharmacokinetics and Pharmacodynamics of Chemotherapeutics within a Spatial SILAC-Labeled Spheroid Model System
Tumors have considerable cellular heterogeneity that is impossible to explore with simple cell cultures. Spheroid cultures contain pathophysiological and chemical gradients similar to in vivo tumors and show complex responses to therapeutics, similar to a tumor. Using pulsed isotopic labels, we demonstrate the pronounced differential response of the proteome to the drug Regorafenib, a multikinase inhibitor, in HCT 116 spheroids.
MS imaging of multicellular tumor spheroids and organoids as an emerging tool for personalized medicine and drug discovery
MS imaging (MSI) is a powerful tool in drug discovery because of its ability to interrogate a wide range of endogenous and exogenous molecules in a broad variety of samples. The impressive versatility of the approach, where almost any ionizable biomolecule can be analyzed, including peptides, proteins, lipids, carbohydrates, and nucleic acids, has been applied to numerous types of complex biological samples.
Electroblotting through a tryptic membrane for LC-MS/MS analysis of proteins separated in electrophoretic gels
Electroblotting through a tryptic membrane for LC-MS/MS analysis of proteins separated in electrophoretic gels Digestion of proteins separated via sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) remains a popular method for protein identification using mass-spectrometry based proteomics. Although robust and routine, the in-gel digestion procedure is laborious and time-consuming.
Considerations for MALDI-Based Quantitative Mass Spectrometry Imaging Studies
Significant advances in mass spectrometry imaging (MSI) have pushed the boundaries in obtaining spatial information and quantification in biological samples. Quantitative MSI (qMSI) has typically been challenging to achieve because of matrix and tissue heterogeneity, inefficient analyte extraction, and ion suppression effects, but recent studies have demonstrated approaches to obtain highly robust methods and reproducible results.
Embracing Rejection: the Key to Success?
Norm Dovichi joined our department during my second year as an assistant professor, and we soon discovered a mutual appreciation for coffee and walking. Over the next seven years, he and I would walk to the local coffee shop almost every day. We would often discuss my efforts to set up a lab that sits at the intersection of cancer biology and analytical chemistry. In particular, I catalogued – in gory detail – each rejected proposal and manuscript.
The Tools of Proteomics
The proteome, which is the set of proteins expressed by a genome, cell, tissue or organism at a given time, presents immense analytical challenges – but offers substantial rewards. Here, current approaches to proteomics, their strengths and their shortcomings, are explored.
Assessing chemotherapeutic effectiveness using a paper-based tumor model
In vitro models for screening new cancer chemotherapeutics often rely on two-dimensional cultures to predict therapeutic potential. Unfortunately, the predictive power of these models is limited, as they fail to recapitulate the complex three-dimensional environments in tumors that promote the development of a chemoresistant phenotype.
Magnetic bead-based peptide extraction methodology for tissue imaging
MALDI-TOF imaging mass spectrometry (IMS) is a common technique used for analyzing tissue samples, as it allows the user to detect multiple different analytes simultaneously. However, the detection and analysis of these analytes may sometimes be hampered due to the presence of contaminants in the tissue microenvironment, which leads to ion suppression.
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