Anabel Guedán

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Abstract The HIV-1 capsid (CA) protein forms the outer shell of the viral core that is released into the cytoplasm upon infection. CA binds various cellular proteins, including CPSF6, that directs HIV-1 integration into speckle associated domains in host chromatin. Upon HIV-1 infection, CPSF6 forms puncta in the nucleus.

ORF6 inhibits the nuclear export of IFN-upregulated mRNA As many mRNA were retained in the nucleus, we decided to investigate whether genes with potential anti-viral functions were among them. Firstly, we identified potential ISGs in our dataset by determining which mRNA species were upregulated by IFN-β treatment in cells transfected with our control plasmid (S5A Fig). We found 134 mRNAs species that were significantly upregulated in either the cytoplasm and/or nucleus after IFN-β treatment.