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Andreas Rohringer

As seen in: BMC Genomics, medRxiv
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Articles

Unsupervised detection of novel SARS-CoV-2 mutations and lineages in wastewater samples using long-read sequencing - BMC Genomics

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By Ignacio M. García, Andreas Rohringer, Elisabeth H. Madslien, Karoline Bragstad, Rasmus K. Riis, Line V. Moen
| BMC Genomics
To evaluate the ability of HERCULES (Fig. 1A and Supplementary Methods) to find and quantify SARS-CoV-2 mutations and lineages in Norwegian wastewater, we regularly collectedwastewater samples between July 26, 2022, and October 30, 2023, from five municipalities in Norway (Bergen, Oslo, Tromsø, Trondheim, and Ullensaker). We extracted the total RNA from the samples and amplified a 1 Kb DNA fragment from the SARS-CoV-2 Spike gene which was then sequenced using Oxford Nanopore Technology (ONT).
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Unsupervised detection of SARS-CoV-2 mutations and lineages in Norwegian wastewater samples using long-read sequencing

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By Ignacio García Verified, Line Victoria Moen, Rasmus Kopperud Riis, Andreas Rohringer
| medRxiv
The authors have declared no competing interest. This study was funded by the Norwegian Institute of Public Health (NIPH) I confirm all relevant ethical guidelines have been followed, and any necessary IRB and/or ethics committee approvals have been obtained.
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