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Andrei Yu Kobitski

As seen in: Wiley Online Library
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Articles

WNT5a export onto extracellular vesicles studied at single‐molecule and single‐vesicle resolution

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By Antonia Schubert, Andrei Yu Kobitski, Matthias Schulz, Ajaree Mongkolsittisilp
| Wiley Online Library Verified
Alix apoptosis-linked gene 2-interacting protein X Arf6 ADP-ribosylation factor 6 CD9/63/81 cluster of differentiation 9/63/81 (Tetraspanins) CM conditioned medium DLS dynamic light scattering Dvl disheveled (e)GFP (Enhanced) Green Fluorescent Protein ESCRT endosomal sorting complex required for transport Evi WNT Cargo receptor evenness interrupted EVs extracellular vesicles Exos exosomes FCS fluorescence correlation spectroscopy FIDA fluorescence intensity distribution analysis FPs...
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A simple route to highly active single-enzyme nanogels

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By Ana Beloqui, Andrei Yu Kobitski, Gerd Ulrich Nienhaus, Guillaume Delaittre
| Royal Society of Chemistry Verified
We have established a simple one-step synthesis of single-enzyme nanogels (SENs), i.e., nanobiocatalysts consisting of an enzyme molecule embedded in a hydrophilic, polymeric crosslinked nanostructure, as a most attractive approach to enhance the stability of enzymes. In contrast to earlier protocols, we demonstrate here that the addition of a small amount of sucrose makes the nanogel formation equally effective as earlier two-step protocols requiring enzyme pre-modification.
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