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BLf was produced by Westland Milk Products company with purity >90%. Iron saturation of BLf (mg iron/100 g of bovine lactoferrin) was detected by atomic absorption spectrophotometry (AA800; PerkinElmer, Waltham, MA, USA). According to GB/T5009.90-2003 standard, FeSO4 was dissolved in deionized water and prepared into 0 mg/L, 1 mg/L, 2 mg/L, 3 mg/L, 4 mg/L, 5 mg/L, respectively.
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