Aneisha Sritharan on Muck Rack

Aneisha Sritharan

As seen in: BioRxiv
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Articles

CRISPR-Cas12a exhibits metal-dependent specificity switching

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By Giang Nguyen, Michael A. Schelling, Kathryn A. Buscher, Aneisha Sritharan
| BioRxiv
Abstract Cas12a is the immune effector of type V-A CRISPR-Cas systems and has been co-opted for genome editing and other biotechnology tools. The specificity of Cas12a has been the subject of extensive investigation both in vitro and in genome editing experiments. However, in vitro studies have often been performed at high magnesium ion concentrations that are inconsistent with the free Mg2+ concentrations that would be present in cells.
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