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Anirban Bardhan

As seen in: Color Research & Application, Wiley Online Library
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Articles

Inverted Molecular Beacons as Reaction-Based Hybridization Probes for Small-Molecule Activation by Nucleic Acid Inputs

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By Cole Emanuelson, Anirban Bardhan, Nicholas Ankenbruck, Jessica Boette
| dx.doi.org
Introduction Click to copy section linkSection link copied! Probes that report on the detection of nucleic acids through the production of a fluorescent signal are important tools for studying biological systems and as molecular diagnostics. (1,2) Many probe detection systems traditionally rely on FRET-type fluorescence quenching to regulate the probe activation.
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Direct Activation of Nucleobases with Small Molecules for the Conditional Control of Antisense Function

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By Anirban Bardhan, Wes Brown, Savannah Albright, Michael Tsang
| Wiley Online Library Verified
Introduction Conditional control of oligonucleotides enables investigation of dynamic cellular processes in a temporal manner through the precise regulation of gene silencing,1 gene editing,2 RNA interference (RNAi),3 RNA splicing,4 and related mechanisms.
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Spatiotemporal Control of CRISPR/Cas9 Function in Cells and Zebrafish using Light‐Activated Guide RNA

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By Wes Brown, Michael Delaney, Wenyuan Zhou, Anirban Bardhan
| Color Research & Application Verified
Research Article Alexander Deiters Corresponding Author E-mail address: deiters@pitt.edu University of Pittsburgh, Chemistry, 219 Parkman Ave, 15260 Pittsburgh, UNITED STATES Search for more papers by this author PDF PDF ShareShare Abstract We developed a new method for conditional regulation of CRISPR/Cas9 activity in mammalian cells and zebrafish embryos via photochemically activated, caged guide RNAs. Caged gRNAs are generated by substituting four nucleobases evenly distributed throughout...
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