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In order to eliminate the toxic effect of chlorogenic acid on DEF and control the effect of chlorogenic acid on DEF within the safe range, the optimal safe concentration of the drug was determined.
The Guizhou strain of duck enteritis virus (DEV-GZ strain), provided by Guizhou Provincial Key Laboratory of Animal Epidemiology and Veterinary Public Health, had a TCID50 of 3.16 × 108 PFU/mL and was titrated by the Reed and Muench method [8] (Reed—Muench). Twenty-four one-day-old Anas platyrhynchos were purchased from Guangdong Mingyan Poultry Industry Co., Ltd., and all were negative for DEV through serological and pathogenic tests.
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