Anna H. Klemm

In the past decade, the speed of innovation in bioimaging has tremendously accelerated, and today’s experiments are producing exponentially increasing amounts of data to be analyzed1,2,3,4. Bioimage analysis has become a crucial step between image acquisition and scientific insight that requires specific data analysis knowledge and experience to ensure the correct interpretation of biological phenomena5.

Abstract Determining the levels of protein–protein interactions is essential for the analysis of signaling within the cell, characterization of mutation effects, protein function and activation in health and disease, among others. Herein, we describe MolBoolean – a method to detect interactions between endogenous proteins in various subcellular compartments, utilizing antibody-DNA conjugates for identification and signal amplification.