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Anna Tsankova

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As seen in: Nature, Cell Press
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Articles by Anna Tsankova

Spatio-temporally separated cortical flows and spindle geometry establish physical asymmetry in fly neural stem cells

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By Anna Tsankova, Tri Pham, Arnaud Monnard, Chantal Roubinet
| Nature Verified
To learn how Myosin dynamics contributes towards sibling cell size asymmetry, we used live cell imaging and measured the relocalization dynamics of Non-muscle Myosin II (visualized with Sqh::GFP19; Myosin (Myo), hereafter) together with the cell cycle marker His2A::mRFP in wild-type fly neuroblasts. We confirmed that Myosin was localized almost uniformly around the cortex by late metaphase12, 18, 20.
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Cell Polarity Regulates Biased Myosin Activity and Dynamics during Asymmetric Cell Division via Drosophila Rho Kinase and Protein Kinase N

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By Anna Tsankova, Tri Pham, David Garcia, Fabian Otte
| Cell Press Verified
Figure 1Biased Myosin Localization Is Dependent on Pins(A–I) Representative image sequence of (A) a wild-type third instar neuroblast expressing Sqh:GFP (Myosin; green in overlay) and Cherry:Jupiter (microtubules [MTs]; white overlay) in (top row), (D) pinsP89/pinsP62 (middle row), and (G) UAS-G?i (bottom row), respectively. Representative kymographs from (B) wild-type, (E) pins mutant, and (H) UAS-G?i-expressing neuroblasts, derived from a line along the apical to basal cortex.
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