Anne-Stephanie Rueff on Muck Rack

Anne-Stephanie Rueff

As seen in: BioRxiv
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Articles

Make-or-break prime editing for bacterial genome engineering

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By Maria-Vittoria Mazzuoli, Axel B. Janssen, Monica Rengifo-Gonzalez, Anne-Stephanie Rueff
| BioRxiv
Abstract CRISPR-Cas9 has revolutionized genome engineering by allowing precise introductions of DNA double-strand breaks (DSBs). However, genome engineering in bacteria is still a complex, multi-step process requiring a donor DNA template for homology-directed repair of DSBs. Prime editing circumvents this need as the repair template is provided within the prime editing guide RNA (pegRNA).
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