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Macrophages migrate in an activation-dependent manner to chemokines involved in neuroinflammation

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By Daphne YS Vogel, Priscilla DAM Heijnen, Marjolein Breur, Anton TJ Tool, Sanquin Research, Landsteiner Laboratory
| jneuroinflammation.com
Peripheral blood mononuclear cells (PBMCs) were isolated from buffy coats (Sanquin Blood Bank, Amsterdam, The Netherlands) obtained from healthy donors who gave informed consent for use of their blood for research purposes. Research was performed with the approval of the Medical Ethical Committee of the VU University Medical Center. PBMCs were isolated using Ficoll density gradient (Lymphoprep™, Axis-Shield, Oslo, Norway).
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