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Brachypodium distachyon - A Useful Model in the Qualification of Mutagen-Induced Micronuclei Using Multicolor FISH

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By Arita Kus, Jolanta Kwasniewska, Robert Hasterok
| PLOS
The treatment of Brachypodium seeds with 3 mM or 4 mM MH caused a prominent clastogenic effect, which was manifested by the presence of micronuclei in the cells at interphase. Analysis of the frequencies of the cells with micronuclei was done using DAPI staining. The frequency of the cells with micronuclei depended on the mutagen concentration and varied from 4.8% for 3 mM MH to 5.6% for 4 mM MH ( Fig 1 ).
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