Augusto Dulanto Chiang

RNA sequencing Twenty-two isolates from the in-vitro evolution experiment were chosen for RNA-seq analysis. The set included 2 × 0, ancestor strain of the hypermutator lineages from the adaptive evolution experiment, and all terminal and intermediate isolates listed in S3 Data for each lineage (2A, 2B, and 2D). The ancestor of the WT lineages in the adaptive evolution experiment, 1 × 0, was also chosen, along with all terminal isolates in 3 WT lineages (1A, 1C, 1D).

Research Article | Host-Microbe Biology Pavel P. Khil, Augusto Dulanto Chiang, Jonathan Ho, Jung-Ho Youn, Jamie K. Lemon, Juan Gea-Banacloche, Karen M. Frank, Mark Parta, Robert A. Bonomo, John P. Dekker Susan Gottesman, Editor DOI: 10.1128/mBio.01822-19 ABSTRACT Strains of Pseudomonas aeruginosa with deficiencies in DNA mismatch repair have been studied in the context of chronic infection, where elevated mutational rates (“hypermutation”) may facilitate the acquisition of antimicrobial resistance.