Ayşe Derya Cavga

Abstract Each human chromosome maintains its individuality during the cell cycle, and occupies a spatially limited volume, termed chromosome territory. Each linear chromosomal DNA is folded into multiple loops in the three-dimensional space, and further organized into densely packed heterochromatin, less dense euchromatin and nucleosome-free regions that are accessible for transcription factor binding.

Given that BRD9 inhibition is most effective in early reprogramming, we next sought to identify the transcriptional effects elicited by different modes of BRD9 inhibition. We performed mRNA-sequencing from fibroblasts treated with BI-7273, I-BRD9, and dBRD9, as well as those expressing Cas9 and BRD9 sgRNA. BRD9 inhibitors differentially downregulated 928, 170, and 577 genes (dBRD9, I-BRD9, and BI-7273, respectively), of which 70 were common to all treatments ( Figures S4 A and S4B).