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Azadeh Ebrahim Habibi on Muck Rack

Azadeh Ebrahim Habibi

As seen in: Nature
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Improvement of split-luciferase complementation through stabilization of NLRP3 PYD fused luciferase fragments: in vitro and in silico studies

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By Mohammad Javan, Saman Hosseinkhani, Fatemeh Vahdani Farahi, Joulia Alizadeh-Rahrovi, Azadeh Ebrahim Habibi
| Nature Verified
Abstract Split-luciferase complementary assay (SLCA) is a key method for studying protein–protein interactions. However, the NLuc and CLuc fusion proteins involved in NLRP3 PYD interactions show reduced luciferase activity due to a loss of structural stability after a freeze–thaw cycle, and homo-oligomerization and misfolding may further compromise their experimental reliability.
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