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TKSM: Highly modular, user-customizable, and scalable transcriptomic sequencing long-read simulator
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scTagger: Fast and accurate matching of cellular barcodes across short- and long-reads of single-cell RNA-seq experiments
Abstract Single-cell RNA sequencing allows for characterizing the gene expression landscape at the cell type level. However, because of its use of short-reads, it is severely limited at detecting full-length features of transcripts such as alternative splicing. New library preparation techniques attempt to extend single-cell sequencing by utilizing both long- and short-reads.
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