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Barbara Conradt on Muck Rack

Barbara Conradt

As seen in: Nature, PLOS Biology
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Tagging of C. elegans apoptosis activator EGL-1 BH3-only reveals CED-9 BCL-2-dependent mitochondrial localization and dynamic control of EGL-1 synthesis and degradation in vivo | Cell Death & Differentiation

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By Yanwen Jiang, Kate Hodgson Verified, Ioannis Segos, Eric J. Lambie, Lumeng Yang, Alan Greig, Barbara Conradt, Minjia Pan
| Nature Verified
Abstract The BH3-only protein EGL-1 is the key activator of apoptosis during C. elegans development. EGL-1 protein is thought to be synthesized predominantly in cells programmed to die and to localize to mitochondria. We used CRISPR-Cas-mediated modification of the egl-1 locus to add the coding sequence for the monomeric StayGold fluorescent protein or 18 copies of the SunTag peptide to the endogenous open reading frame.
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